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mark72
USA
22 Posts |
 Posted - 12/31/2015 : 07:19:53
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Topically applied Melaleuca alternifolia (tea tree) oil causes direct anti-cancer cytotoxicity in subcutaneous tumour bearing mice.
Ireland DJ1, Greay SJ, Hooper CM, Kissick HT, Filion P, Riley TV, Beilharz MW.
Author information
· 1School of Pathology and Laboratory Medicine (M504), Faculty of Medicine, Dentistry and Health Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia. demelza.ireland@uwa.edu.au
Abstract
BACKGROUND:
Melaleuca alternifolia (tea tree) oil (TTO) applied topically in a dilute (10%) dimethyl sulphoxide (DMSO) formulation exerts a rapid anti-cancer effect after a short treatment protocol. Tumour clearance is associated with skin irritation mediated by neutrophils which quickly and completely resolves upon treatment cessation.
OBJECTIVE:
To examine the mechanism of action underlying the anti-cancer activity of TTO.
METHODS:
Immune cell changes in subcutaneous tumour bearing mice in response to topically applied TTO treatments were assessed by flow cytometry and immunohistochemistry. Direct cytotoxicity of TTO on tumour cells in vivo was assessed by transmission electron microscopy.
RESULTS:
Neutrophils accumulate in the skin following topical 10% TTO/DMSO treatment but are not required for tumour clearance as neutrophil depletion did not abrogate the anti-cancer effect. Topically applied 10% TTO/DMSO, but not neat TTO, induces an accumulation and activation of dendritic cells and an accumulation of T cells. Although topical application of 10% TTO/DMSO appears to activate an immune response, anti-tumour efficacy is mediated by a direct effect on tumour cells in vivo. The direct cytotoxicity of TTO in vivo appears to be associated with TTO penetration.
CONCLUSION:
Future studies should focus on enhancing the direct cytotoxicity of TTO by increasing penetration through skin to achieve a higher in situ terpene concentration. This coupled with boosting a more specific anti-tumour immune response will likely result in long term clearance of tumours.
Copyright © 2012 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.
PMID: 22727730 [PubMed - indexed for MEDLINE]
http://www.ncbi.nlm.nih.gov/pubmed/22727730
While there are well doccumented:
In vitro experiments
Tea Tree Oil inhibits cellular viability
TTO inhibited cellular viability of the two tumor cell lines studied AE17 Mesothelioma and B16
Melanoma, in a dose-and time-dependent manner as measured by the MTT assay.
TTO and Terpinen-4-ol induce cell death by necrosis and apoptosis.
In vivo experiments
10% TTO inhibits AE17 and B16 tumor growth and induces AE17 tumor
regression
Mice with established (~9mm2) AE17 and B16 (~15mm2) (Figure 26) implanted
subcutaneous tumors, were treated topically with 50ìl 10% TTO in DMSO or DMSO alone (solvent control) for 4 days.
TTO completely significantly (P<0.05) inhibited mesothelioma (AE17) tumor growth and
significantly (P<0.05) reduced tumor area by ~100% during treatment (2 groups of 3 mice).
TTO was least efficacious against the normal human fibroblast cells HF32, with IC50 values of 0.08%±0.01 (24h), 0.07%±0.02 (48h) and 0.07%±0.01 (72h). Although there was a dose dependent decrease in cellular viability, prolonged exposure time from 24h to 48h to 72h had no significant effect on the cells. Only concentrations of TTO (0.1%) after 24h, 0.06%-0.1%) after 48h and (0.08-0.1%) after 72h significantly (P<0.05) reduced HF32 cell viability.
I decided to experiment to myself treating a Squamous Carcinoma that went bad after biopsy and a surgically treatment, and finaly over a 1/8" black spot that refused to go and started growing up over the skin.
After getting positive results I started taking pictures that I can share, but this is my experience, my testimony, everybody is different, there are many things that may go wrong. The devil stay on detals. There are too many steps to follow up and have to be done with a very strict regimen. The size, age and type of skin cancer modify the treatment.
My Goal is:
To keep the concentration of TTO in DMSO, as high as possible, to induce tumor cell death by necrosis and apoptosis, but to minimize the effect against normal human fibroblast cells.
The indicator is the rate of formation of necrotic cells.
The treated area is monitored every 8 to 6 hours, and necrotic cells are removed with warm, concentrated chamomile solution, via cotton balls. This process takes at least 10 minutes.
The formation of scabs compromises the treatment, because it blocks the penetration of TTO, and creates pockets, prone of the secondary infections.
This is a dose-and time-dependent manner treatment.
Depending how the treatment progress, the concentration of TTO variates between 5% to 15%, and the frequency of applications every 6hours to 12 hours. Collateral damages cannot be avoided, but they are minimal.
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Dougrun
92 Posts |
Posted - 12/31/2015 : 12:55:25
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| clearly dmso is the key to this. I created a good size crater where by basal cell was using frankincense oil and diluted dmso. However, after healing, the basal cell came back. |
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mark72
USA
22 Posts |
Posted - 01/01/2016 : 00:02:03
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https://en.wikipedia.org/wiki/Dimethyl_sulfoxide
Dimethyl sulfoxide (DMSO) is an organosulfur compound with the formula (CH3)2SO. This colorless liquid is an important polar aprotic solvent that dissolves both polar and nonpolar compounds and is miscible in a wide range of organic solvents as well as water.
Use of DMSO in medicine dates from around 1963, when an Oregon Health & Science University Medical School team, headed by Stanley Jacob, discovered it could penetrate the skin and other membranes without damaging them and could carry other compounds into a biological system. In medicine, DMSO is predominantly used as a topical analgesic, a vehicle for topical application of pharmaceuticals, as an anti-inflammatory, and an antioxidant. Because DMSO increases the rate of absorption of some compounds through organic tissues, including skin, it is used in some transdermal drug delivery systems
It is frequently compounded with antifungal medications, enabling them to penetrate not just skin but also toenails and fingernails.
DMSO - The Magic Bullet For Cancer.
http://www.cancertutor.com/dmso/
DMSO and Vitamin C - The Magic Duo for Cancer Treatment
http://preventdisease.com/news/13/082213_DMSO-and-Vitamin-C-The-Magic-Duo-for-Cancer-Treatment-That-Frightens-FDA-Conventional-Medicine.shtml
DMSO CANCER TREATMENT: MIRACLE OR SCAM?
http://clarkfreelancewriting.com/documents/DMSO-cancer-treatment.pdf
https://microbewiki.kenyon.edu/index.php/Tea_Tree_Oil_Treatment_of_MRSA
Tea Tree Oil Treatment of MRSA
In vitro Effectiveness
Figure 4. The in vitro and clinical effectiveness of tea tree oil (TTO), as described by May et al.,Caelli et al. and Dryden et al.. Image created by Karen Leung.
https://microbewiki.kenyon.edu/index.php/File:Potency_TTO.png
The efficacy of tea tree oil (TTO) against MRSA has been demonstrated in laboratory studies using cultured S. aureus samples. An in vitro study by May et al. showed that 99.9% of the MRSA isolate was killed by TTO within 4 hours and that all was eradicated after 6 hours of continuous exposure to 5% TTO. These results suggest that TTO's antimicrobial properties make this a good agent to control MRSA and reduce its transmission among humans.
Clinical Efficiency
Figure 4. Impetigo, a skin infection resulting from staph infection. Dryden et al. found that tea tree oil could effectively treat a similar MRSA-related ailment.
The efficiency of TTO in eradicating MRSA cultures in vitro led to clinical testing with human patients using products enhanced with TTO. TTO may be added to commercial products, such as body wash, skin cream, and nasal ointment due to its antimicrobial properties. The clinical efficacy of TTO body wash and creams as a decolonization agent for MRSA has been supported by Caelli et al. and Dryden et al.. Both researchers found that TTO treatment is as effective as the standard treatment regimen, indicating that TTO treatment may enhance a MRSA eradication regimen.
Dermal Toxicity
Despite characterizing TTO’s antimicrobial properties, researchers have not done much work on the safety and toxicity of using TTO topically. The rationale for continued topical use of TTO primarily rests on anecdotal evidence of its safe usage at low concentrations. No human deaths due to TTO exposure have been reported. Though there are few substantial scientific studies regarding TTO’s safety, TTO can cause irritant or allergic reactions when applied to the skin. More concrete evidence of TTO’s toxicity would be necessary in order to determine its optimal concentration for safe usage.
Based on my experience:
While a 5% TTO solution is a sub-lethal concentration of TTO against MRSA infection, Melanoma, and Squamous Carcinoma, a 10% TTO in 99.9% DMSO (1 Vol TTO / 9 Vol Concentrated DMSO) solution proved to be a lethal concentration of TTO against bacterial infections and Squamous Carcinoma.
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Edited by - mark72 on 01/01/2016 06:23:18 |
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Topically applied Melaleuca alternifolia (tea tree
